KMID : 1100220160150040122
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Dementia and Neurocognitive Disorders 2016 Volume.15 No. 4 p.122 ~ p.128
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The Correlation Study between Plasma A¥â Proteins and Cerebrospinal Fluid Alzheimer¡¯s Disease Biomarkers
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Shin Ho-Sik
Lee Seung-Keun Kim Saeromi Kim Hyung-Jun Chae Won-Seok Park Sun-Ah
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Abstract
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Background and Purpose : Alzheimer¡¯s disease (AD) is characterized by the accumulation of amyloid-¥â proteins (A¥â). In this study we explored the correlation of plasma A¥â40 and A¥â42 concentrations with A¥â42, total tau (tTau) and phosphorylated tau 181 (pTau181) levels in cerebrospinal fluid (CSF) in AD and control subjects to further understand the characteristics of plasma A¥â proteins levels.
Methods : The consecutive subjects (44 AD and 47 controls) in this study were recruited. The plasma levels of A¥â40 and A¥â42 were measured using a commercially available sandwich enzyme-linked immunosorbent assay (ELISA) kits. And the corresponding CSFs were analyzed in terms of A¥â42, tTau and pTau181 concentrations using INNOTEST ELISA kits. Further, the albumin levels were measured both in serum and CSF and albumin ratio was obtained to check the integrity of blood-brain barrier.
Results : CSF A¥â42 concentrations were significantly decreased while tTau and pTau181 levels were significantly increased in AD subjects. The plasma levels of A¥â42 were significantly lower (p=0.007), while the A¥â40/A¥â42 ratio was significantly higher (p<0.001) in AD patients than in controls. The overall plasma A¥â42 levels showed a positive correlation with those of CSF A¥â42 (p=0.001), but not with the others in CSF. In subgroup analysis, the CSF A¥â42 demonstrated positive correlation with not only plasma A¥â42 but also A¥â40 levels in controls. However, no significant relationship was noted between plasma and CSF A¥â proteins in AD group.
Conclusions : The plasma A¥â42 and A¥â40 concentrations were shown to have a close relationship with CSF A¥â42 levels in controls, but not in AD subjects. Our results suggest that the correlation between plasma A¥â40 and CSF A¥â42 levels is perturbed in AD.
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KEYWORD
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Alzheimer¡¯s disease, amyloid ¥â proteins, biomarker, blood, cerebrospinal fluid
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